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Goat Anti-TOM1L2 Antibody (EB06556)

Related Products: EBP06556 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06556 Goat Anti-TOM1L2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06556 1
EB06556 (0.03µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06556 2
EB06556 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TOM1L2, FLJ32746, target of myb1-like 2 (chicken), target of myb1 (chicken) homolog-like 1, target of myb1 (chicken) homolog-like 2
Official Symbol: TOM1L2
Accession Number(s): NP_001028723.1; NP_001076437.1
Human GeneID(s): 146691
Important Comments: This antibody is expected to recognise both reported isoforms.

Immunogen

Peptide with sequence CRKKPERSEDALFAL, from the C Terminus of the protein sequence according to NP_001028723.1; NP_001076437.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 60kDa band observed in Human Fibroblast A431 lysates (calculated MW of 51.8kDa according to NP_653279). Recommended concentration: 0.1-1µg/ml.
IHC: In paraffin embedded Human Skeletal Muscle shows variable staining from fibre to fibre. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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