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Goat Anti-SRD5A1 / 5-alpha reductase 1 Antibody (EB06638)

Related Products: EBP06638 - Immunizing Peptide, EBP09209 - Immunizing Peptide, Goat Anti-SRD5A1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06638 Goat Anti-SRD5A1 / 5-alpha reductase 1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: [email protected]
www.arp1.com
EB06638 1
EB06638 (4µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06638 2
EB06638 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SRD5A1, steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1), 5-alpha reductase, steroid 5-alpha-reductase type I, 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1, steroid-5-alpha-reductase 1
Official Symbol: SRD5A1
Accession Number(s): NP_001038.1
Human GeneID(s): 6715

Immunogen

Peptide with sequence ATATGVAEERLLC, from the N Terminus of the protein sequence according to NP_001038.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: In paraffin embedded Human Prostate shows reticulate staining in the cytoplasm of secretory cells in the gland. Recommended concentration: 4-6µg/ml. Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody (previous batch) has been successfully used in WB in Human:
Kapp FG, Sommer A, Kiefer T, Dölken G, Haendler B.
5-alpha-reductase type I (SRD5A1) is up-regulated in non-small cell lung cancer but does not impact proliferation, cell cycle distribution or apoptosis.
Cancer Cell Int. 2012 Jan 18;12(1):1. doi: 10.1186/1475-2867-12-1.
PMID: 22257483

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