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Goat Anti-DYX1C1 (Isoform a) Antibody (EB06656)

Related Products: EBP06656 - Immunizing Peptide, EBP07254 - Immunizing Peptide, Goat Anti-DYX1C1 / EKN1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06656 Goat Anti-DYX1C1 (Isoform a) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB06656 1
EB06656 staining (0.1µg/ml) of COS1 cell lysates: untransfected (left lane) and transfected with full length recombinant Human DYX1C1 (right lane). Data kindly provided by Wang and LoTurco, University of Connecticut, USA.
EB06656 2
EB06656 (5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DYX1C1, DYX1, EKN1, DYXC1, FLJ37882, dyslexia susceptibility 1 candidate 1, MGC70618, RD
Official Symbol: DNAAF4
Accession Number(s): NP_570722.2
Human GeneID(s): 161582
Non-Human GeneID(s): 67685 (mouse)
Important Comments: This antibody is expected to recognise only one of the three reported isoforms (NP_570722.2, isoform a).

Immunogen

Peptide with sequence C-KIRNVIQGTELKS, from the C Terminus of the protein sequence according to NP_570722.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1µg/ml antibody staining. In addition, a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA). The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.
IHC: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody has been successfully used (in WB on Rat Brain (24kDa and 48kDa)) in the following paper:
Wang Y, Paramasivam M, Thomas A, Bai J, Kaminen-Ahola N, Kere J, Voskuil J, Rosen GD, Galaburda AM, Loturco JJ.
DYX1C1 functions in neuronal migration in developing neocortex.
Neuroscience. 2006 Dec 1;143(2):515-522. Epub 2006 Sep 20.
PMID: 16989952

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