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Goat Anti-SNAP25, Biotinylated Antibody (EB06738-B)

Related Products: EBP06738-B - Immunizing Peptide, EBP06738 - Immunizing Peptide, Goat Anti-SNAP25 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06738-B Goat Anti-SNAP25, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06738-B 1
Biotinylated EB06738 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: SNAP25, synaptosome associated protein 25kDa, CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2, resistance to inhibitors of cholinesterase 4 homolog, synaptosomal-associated protein, 25kDa
Official Symbol: SNAP25
Accession Number(s): NP_003072.2; NP_570824.1
Human GeneID(s): 6616
Non-Human GeneID(s): 20614 (mouse), 25012 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1

Immunogen

Peptide with sequence C-DEANQRATKMLGSG., from the C Terminus of the protein sequence according to NP_003072.2; NP_570824.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 26kDa band observed in Rat Brain lysates (calculated MW of 23.3kDa according to NP_003072.2 and NP_570824.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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