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Goat Anti-LRP5 Antibody (EB06771)

Related Products: EBP06771 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06771 Goat Anti-LRP5 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06771 1
EB06771 (1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06771 2
EB06771 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: LRP5, low density lipoprotein receptor-related protein 5, HGNC:6697, BMND1, HBM, LR3, LRP7, OPPG, OPS, VBCH2, low density lipoprotein receptor-related protein 7, osteoporosis pseudoglioma syndrome, EVR1, EVR4, exudative vitreoretinopathy 1
Official Symbol: LRP5
Accession Number(s): NP_002326.2
Human GeneID(s): 4041

Immunogen

Peptide with sequence C-ERVEKTTGDKRT, from the internal region of the protein sequence according to NP_002326.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 180kDa band observed in lysates of cell line HepG2 (calculated MW of 179kDa according to NP_002326.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver for this particular batch.
IHC: In paraffin embedded Human Liver shows weak cytoplasm staining in some hepatocytes. Recommended concentration: 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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