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Code Name Applications Availability Size Tested species Price Grade  
EB06795 Goat Anti-Arginase I Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human, Pig $450.00 Elite_Plus

Ordering Information for

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06795 1
EB06795 (0.3µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06795 2
EB06795 (0.01µg/ml) staining of Human (A) and (0.03ug/ml) Pig (B) Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06795 3
EB06795 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06795 4
EB06795 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06795 5
EB06795 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARG1, arginase, liver, HGNC:663, A-I, arginase, type I, arginase 1, liver-type arginase, type I arginase
Official Symbol: ARG1
Accession Number(s): NP_000036.2; NP_001231367.1; NP_001355949.1
Human GeneID(s): 383
Non-Human GeneID(s): 11846 (mouse), 29221 (rat)
Important Comments: Please note that ARG1 is not expressed in Human M2 macrophages, but it is in other species (eg rodents).

Immunogen

Peptide with sequence CFGLAREGNHKPID, from the C Terminus of the protein sequence according to NP_000036.2; NP_001231367.1; NP_001355949.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 38kDa band observed in lysates of cell line HepG2, and approx. 37kDa in Human and Pig Liver lysates (calculated MW of 34.7kDa according to Human NP_000036.2 and 35.0kDa according to Pig NP_999213.1). Recommended concentration: 0.01-0.3µg/ml. Primary incubation 1 hour at room temperature.

Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.


IHC: Paraffin embedded Human Liver. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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