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Goat Anti-Osteopontin / SPP1 Antibody (EB06825)

Related Products: EBP06825 - Immunizing Peptide, Goat Anti-Osteopontin / SPP1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06825 Goat Anti-Osteopontin / SPP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06825 1
EB06825 (0.1µg/ml) staining of lysates of cell line MOLT4 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1, SPP1/CALPHA1 fusion, bone sialoprotein I, early T-lymphocyte activation 1, osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
Official Symbol: SPP1
Accession Number(s): NP_001035147.1; NP_000573.1; NP_001035149.1
Human GeneID(s): 6696
Non-Human GeneID(s): 20750 (mouse)

Immunogen

Peptide with sequence C-DPKSKEEDKHLK, from the C Terminus of the protein sequence according to NP_001035147.1; NP_000573.1; NP_001035149.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in lysates of cell line MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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