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Goat Anti-Complement factor H Antibody (EB06848)

Related Products: EBP06848 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06848 Goat Anti-Complement factor H Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Ph. (800) 832-2611
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EB06848 1
EB06848 staining (0.03µg/ml) of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06848 2
EB06848 staining (0.3µg/ml) of K562 cell lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
EB06848 3
EB06848 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06848 4
EB06848 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: complement factor H, HGNC:4883, CFH, CFHL3, FHL1, HF, HF1, HF2, HUS, H factor 1 (complement), H factor 2 (complement), H factor-1 (complement), factor H-like 1, AHUS1, ARMD4, ARMS1, FH, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H, complement factor H, isoform b, factor H, factor H-like 1
Official Symbol: CFH
Accession Number(s): NP_000177.2
Human GeneID(s): 3075
Important Comments: This antibody is expected to recognize isoform a (NP_000177.2) only.

Immunogen

Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150kDa band observed in Human Kidney lysates and in lysates of cell line K562 (calculated MW of 139kDa according to NP_000177.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.3µg/ml. Primary incubation 1 hour art room temperature.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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