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Goat Anti-Ghrelin preproprotein Antibody (EB06876)

Related Products: EBP06876 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06876 Goat Anti-Ghrelin preproprotein Antibody Pep-ELISA, IHC, ICC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06876 1
EB06876 (2µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06876 2
EB06876 (2µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GHRL, MTLRP, ghrelin, ghrelin precursor, motilin-related peptide, preproghrelin, ghrelin/obestatin prepropeptide, obestatin, ghrelin/obestatin preprohormone, OTTHUMP00000207794;, OTTHUMP00000207795, OTTHUMP00000207796, OTTHUMP00000207798, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin
Official Symbol: GHRL
Accession Number(s): NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1
Human GeneID(s): 51738
Important Comments: This antibody is expected to recognise all reported isoforms.

Immunogen

Peptide with sequence C-QDILWEEAKEAPADK, from the C Terminus of the protein sequence according to NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: In paraffin embedded Human Placenta shows staining of trophoblast cytoplasm. Recommended concentration: 2-5µg/ml. Paraffin embedded Human Colon. Recommended concentration: 2ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in ICC on Rat:
Stoyanova II, Wiertz RW, Rutten WL.
Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex.
Regul Pept. 2009 Nov 27;158(1-3):86-90.
PMID: 19540881

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