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Goat Anti-GGA3 Antibody (EB06885)

Code Name Applications Availability Size Tested species Price Grade  
EB06885 Goat Anti-GGA3 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB06885 1
EB06885 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: GGA3, golgi associated, gamma adaptin ear containing, ARF binding protein 3, HGNC:17079, KIAA0154, ADP-ribosylation factor binding protein 3, Golgi-localized, gamma ear-containing, ARF-binding protein 3
Official Symbol: GGA3
Accession Number(s): NP_619525.1; NP_054720.1
Human GeneID(s): 23163
Important Comments: This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720)

Immunogen

Peptide with sequence C-EQLSTEVGEVDQ, from the C Terminus of the protein sequence according to NP_619525.1; NP_054720.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave an approx 65kDa band in Mouse and Rat Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.0kDa according to NP_766636.2 and 70.2kDa according to NP_001238996.1. The 65kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog