Change country
Home

Goat Anti-ZASP/ CYPHER / LDB3 Antibody (EB06923)

Related Products: EBP06923 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06923 Goat Anti-ZASP/ CYPHER / LDB3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06923 1
EB06923 (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06923 2
EB06923 (6µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06923 3
EB06923 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: LDB3, ZASP, LIM domain binding 3, HGNC:15710, KIAA01613, KIAA0613, ORACLE, PDLIM6, PDZ and LIM domain 6, Z-band alternatively spliced PDZ-motif, CYPHER, FLJ35865, ldb3z1, ldb3z4
Official Symbol: LDB3
Accession Number(s): NP_009009.1; NP_001073583.1; NP_001073584.1; NP_001073585.1
Human GeneID(s): 11155

Immunogen

Peptide with sequence C-KSQNKPEDEADE, from the internal region of the protein sequence according to NP_009009.1; NP_001073583.1; NP_001073584.1; NP_001073585.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Western Blot experiments gave bands at approx 90kDa and 35kDa in Human Skeletal Muscle lysate after 1µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.1-1µg/ml.
IHC: Paraffin embedded Human Heart. Recommended concentration: 6-7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Product Reviews

Please login to review this product.