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Goat Anti-14-3-3 sigma / Stratifin Antibody (EB07061)

Related Products: EBP07061-B - Immunizing Peptide, EBP07061 - Immunizing Peptide, Goat Anti-14-3-3 sigma / Stratifin, Biotinylated Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07061 Goat Anti-14-3-3 sigma / Stratifin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07061 1
EB07061 (0.03µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07061 2
EB07061 (0.03µg/ml) staining of A431 (A) and U2OS (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07061 3
EB07061 (0.03µg/ml) staining of Mouse (A) and Rat (B) Skin lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07061 4
EB07061 (2µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: 14-3-3sigma, stratifin, HGNC:10773, SFN, YWHAS
Official Symbol: SFN
Accession Number(s): NP_006133.1
Human GeneID(s): 2810
Non-Human GeneID(s): 55948 (mouse), 313017 (rat)

Immunogen

Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 26-28kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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