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Goat Anti-STMN1 / Stathmin 1 Antibody (EB07115)

Related Products: EBP07115 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07115 Goat Anti-STMN1 / Stathmin 1 Antibody Pep-ELISA, WB, EIA, IHC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07115 1
EB07115 (1µg/ml) staining of Human Testis Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07115 2
EB07115 (3.75µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07115 3
EB07115 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: STMN1, stathmin 1/oncoprotein 18, LAP18, Lag, OP18, PP17, PP19, PR22, SMN, Pr22 protein, leukemia-associated phosphoprotein p18, metablastin, oncoprotein 18, phosphoprotein 19, prosolin, stathmin 1, stathmin 1 variant
Official Symbol: STMN1
Accession Number(s): NP_005554.1; NP_981944.1; NP_981946.1
Human GeneID(s): 3925
Important Comments: This antibody is expected to cross-react with isoform 1 a (NP_005554.1; NP_981944.1; NP_981946.1) only. The three reported isoforms (NP_005554.1, NP_981944.1 and NP_981946.1) represent identical protein.

Immunogen

Peptide with sequence C-KNKESKDPADETEAD, from the C Terminus of the protein sequence according to NP_005554.1; NP_981944.1; NP_981946.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 17kDa band observed in Human Testis and Mouse Brain lysates (calculated MW of 17.3kDa according to NP_005554.1, NP_981944.1 and NP_981946.1). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Thymus and Tonsil. Recommended concentration: 3.75µg/ml.

ELISA: This antibody has been successfully used in ELISA as described in the following paper: Song et al, Aging Cell. 2010 Aug;9(4):607-15; PMID: 20560902.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

Specific References

This antibody has been successfully used in the following paper:
Watabe-Rudolph M, Song Z, Lausser L, Schnack C, Begus-Nahrmann Y, Scheithauer MO, Rettinger G, Otto M, Tumani H, Thal DR, Attems J, Jellinger KA, Kestler HA, von Arnim CA, Rudolph KL.
Chitinase enzyme activity in CSF is a powerful biomarker of Alzheimer disease.
Neurology. 2012 Feb 21;78(8):569-77.
PMID: 22323746

This antibody has been successfully used in ELISA:
Song Z, von Figura G, Liu Y, Kraus JM, Torrice C, Dillon P, Rudolph-Watabe M, Ju Z, Kestler HA, Sanoff H, Lenhard Rudolph K.
Lifestyle impacts on the aging-associated expression of biomarkers of DNA damage and telomere dysfunction in human blood.
Aging Cell. 2010 Aug;9(4):607-15.
PMID: 20560902

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