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Goat Anti-STAM2 Antibody (EB07213)

Related Products: EBP07213 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07213 Goat Anti-STAM2 Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07213 1
EB07213 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07213 2
EB07213 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07213 3
EB07213 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: STAM2, signal transducing adaptor molecule (SH3 domain and ITAM motif) 2, HGNC:11358, DKFZp564C047, Hbp, STAM2A, STAM2B, STAM-like protein containing SH3 and ITAM domains 2, signal transducing adaptor molecule 2
Official Symbol: STAM2
Accession Number(s): NP_005834.3
Human GeneID(s): 10254
Non-Human GeneID(s): 56324 (mouse)
Important Comments: No cross-reaction expected with STAM1.

Immunogen

Peptide with sequence C-QSIDPTDSKPDSQD, from the internal region of the protein sequence according to NP_005834.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 70kDa band observed in Human Spleen lysates and in preliminary testing of HeLa cell lysate (calculated MW of 58.2kDa according to NP_005834.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml.. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of K562 and HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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