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Goat Anti-EXOC7 Antibody (EB07225)

Related Products: EBP07225 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07225 Goat Anti-EXOC7 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07225 1
EB07225 (0.5µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07225 2
EB07225 (0.5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EXOC7, exocyst complex component 7, HGNC:23214, 2-5-3p, EXO70, Exo70p, YJL085W
Official Symbol: EXOC7
Accession Number(s): NP_001013861.1; NP_056034.2; NP_001138769.1; NP_001138771.1; NP_001138770.1
Human GeneID(s): 23265
Important Comments: This antibody is expected to recognise all five reported isoforms (NP_001013861.1; NP_056034.2; NP_001138769.1; NP_001138771.1; NP_001138770.1).

Immunogen

Peptide with sequence C-DNIKNDPDKEYN, from the internal region of the protein sequence according to NP_001013861.1; NP_056034.2; NP_001138769.1; NP_001138771.1; NP_001138770.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70kDa band observed in Human Lung lysates (calculated MW of 74.7kDa according to NP_056034.2). Recommended concentration: 0.5-1.5µg/ml.
IHC: In paraffin embedded Human Adrenal Gland shows staining of cytoplasm membranes and cytoplasm of secretory cells in the medulla . Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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