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Goat Anti-WNT3 Antibody (EB07253)

Related Products: EBP07253 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07253 Goat Anti-WNT3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB07253 1
EB07253 (1µg/ml) staining of lysate of cell line HEK293 (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07253 2
EB7253 (2µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: WNT3, wingless-type MMTV integration site family, member 3, INT4, MGC131950, MGC138321, MGC138323, WNT-3 proto-oncogene protein precursor
Official Symbol: WNT3
Accession Number(s): NP_110380.1
Human GeneID(s): 7473
Non-Human GeneID(s): 22415 (mouse), 24882 (rat)

Immunogen

Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration: 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody (previous batch) has been successfully used in IHC on Rat:
Wakabayashi T, Hidaka R, Fujimaki S, Asashima M, Kuwabara T.
Diabetes Impairs Wnt3 Protein-induced Neurogenesis in Olfactory Bulbs via Glutamate Transporter 1Inhibition.
J Biol Chem. 2016 Jul 15;291(29)
PMID: 27226528

This antibody (previous batch) has been successfully used in IHC on Mouse:
Fujimaki S, Hidaka R, Asashima M, Takemasa T, Kuwabara T.
Wnt protein-mediated satellite cell conversion in adult and aged mice following voluntary wheel running.
J Biol Chem. 2014 Mar 14;289(11):7399-412.
PMID: 24482229

This antibody (previous batch) has been successfully used in ICC/IF on Mouse:
Okamoto M, Inoue K, Iwamura H, Terashima K, Soya H, Asashima M, Kuwabara T.
Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis.
FASEB J. 2011 Jul 11.
PMID: 21746862

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