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Goat Anti-Chat (mouse) Antibody (EB07255)

Related Products: EBP07255 - Immunizing Peptide, Goat Anti-Chat (mouse) Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB07255 Goat Anti-Chat (mouse) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc
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EB07255 1
EB07255 (1µg/ml) staining of Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07255 2
EB07255 (1:2000) staining of PFA-perfused cryosection of Mouse Forebrain. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
Click here to download PDF datasheet.

Target Protein

Principal Names: Chat, choline acetyltransferase, B230380D24Rik
Official Symbol: Chat
Accession Number(s): NP_034021.1
Non-Human GeneID(s): 12647 (mouse), 432359 (rat)
Important Comments: This antibody is NOT expected to cross-react to Human CHAT.

Immunogen

Peptide with sequence C-KERARGPTKPSNLD, from the C Terminus of the protein sequence according to NP_034021.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat

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