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Goat Anti-Chat (mouse) Antibody - Trial (EB07255-T)

Code Name Applications Availability Size Species reactivity Price Grade  
EB07255-T Goat Anti-Chat (mouse) Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Mouse, Rat $105.00 Elite

Ordering Information for

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

TestShot
EB07255-T 1
EB07255 (0.2µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: B230380D24Rik, choline acetyltransferase, Chat
Official Symbol: Chat
Accession Number(s): NP_034021.1
Non-Human GeneID(s): 12647 (mouse), 432359 (rat)
Important Comments: This antibody is NOT expected to cross-react to Human CHAT.

Immunogen

Peptide with sequence C-KERARGPTKPSNLD, from the C Terminus of the protein sequence according to NP_034021.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75-80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration: 0.2-0.5µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat

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