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Goat Anti-Ezrin / villin 2 Antibody (EB07305)

Related Products: EBP07305 - Immunizing Peptide, Goat Anti-Ezrin / villin 2 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB07305 Goat Anti-Ezrin / villin 2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07305 1
EB07305 (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07305 2
EB07305 (3.75µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07305 3
EB07305 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: villin 2 (ezrin), ezrin, VIL2, CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, cytovillin, villin 2
Official Symbol: EZR
Accession Number(s): NP_003370.2
Human GeneID(s): 7430
Non-Human GeneID(s): 22350 (mouse), 54319 (rat)

Immunogen

Peptide with sequence C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 80kDa band observed in Human Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Colon and Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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