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Goat Anti-PIK3CA / P110alpha Antibody (EB07512)

Related Products: EBP07512 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07512 Goat Anti-PIK3CA / P110alpha Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB07512 1
EB07512 (2.5µg/ml) staining of paraffin embedded Human Brain (Cerebellar Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07512 2
EB07512 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PIK3CA, p110-alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide, MGC142161, MGC142163, PI3K, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
Official Symbol: PIK3CA
Accession Number(s): NP_006209.2
Human GeneID(s): 5290
Non-Human GeneID(s): 18706 (mouse), 170911 (rat)

Immunogen

Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3µg/ml. Paraffin embedded Human Heart. Recommended concentration: 5µg/ml. This antibody has been successfully used in IHC on Human in the following paper: Abubaker J et al. (2009) PMID: 19638206.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in IHC on Human:
Abubaker J, Bavi P, Al-Haqawi W, Jehan Z, Munkarah A, Uddin S, Al-Kuraya KS.
PIK3CA alterations in Middle Eastern ovarian cancers.
Mol Cancer. 2009 Jul 28;8:51.
PMID: 19638206

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