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Code Name Applications Availability Size Tested species Price Grade  
EB07520 Goat Anti-FOXP2 (internal) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07520 1
EB07520 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07520 2
EB07520 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXP2, forkhead box P2, CAGH44, DKFZp686H1726, SPCH1, TNRC10, CAG repeat protein 44, forkhead/winged-helix transcription factor, speech and language disorder 1, trinucleotide repeat containing 10
Official Symbol: FOXP2
Accession Number(s): NP_055306.1; NP_683696.2; NP_683697.2; NP_683698.2; NP_001166237.1
Human GeneID(s): 93986
Non-Human GeneID(s): 114142 (mouse)
Important Comments: This antibody is expected to recognize isoform I (NP_055306.1), isoform II (NP_683696.2), isoform IV (NP_683698.2) and isoform V (NP_001166237.1).

Immunogen

Peptide with sequence C-DEVEYQKRRSQKIT, from the internal region of the protein sequence according to NP_055306.1; NP_683696.2; NP_683697.2; NP_683698.2; NP_001166237.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary testing showed an approx 90kDa band in Human Cerebellum lysate and an approx 75kDa band in Rat Brain lysate after 0.3ug/ml staining. An additional band of unknown identity was also consistently observed at 28-30kDa and was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cat, Dog, Pig, Cow, Zebrafish

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