Change country
Home

Goat Anti-Semaphorin 3E (internal) Antibody (EB07541)

Related Products: EBP06661 - Immunizing Peptide, EBP07541 - Immunizing Peptide, Goat Anti-SEMA3E Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07541 Goat Anti-Semaphorin 3E (internal) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07541 1
EB07541 (4µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SEMA3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, KIAA0331, M-SEMAH, M-SemaK, M-sema H, SEMAH, coll-5, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E, semaphorin 3E
Official Symbol: SEMA3E
Accession Number(s): NP_036563.1; NP_001171600.1
Human GeneID(s): 9723
Non-Human GeneID(s): 20349 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).

Immunogen

Peptide with sequence C-NGGRYGTTKDYPDD, from the internal region of the protein sequence according to NP_036563.1; NP_001171600.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Product Reviews

Please login to review this product.