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Goat Anti-AKT1 Antibody (EB07691)

Related Products: EBP06875 - Immunizing Peptide, EBP07691 - Immunizing Peptide, Goat Anti-AKT1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07691 Goat Anti-AKT1 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07691 1
EB07691 (0.3µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07691 2
EB07691 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Microtubule/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07691 3
EB07691 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: AKT1, v-akt murine thymoma viral oncogene homolog 1, AKT, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha
Official Symbol: AKT1
Accession Number(s): NP_005154.2; NP_001014431.1; NP_001014432.1
Human GeneID(s): 207
Important Comments: All variants represent identical protein (NP_005154.2, NP_001014431.1, NP_001014432.1). This antibody is NOT expected to cross-react with AKT2. It may recognize AKT1 in Rat and Dog.

Immunogen

Peptide with sequence C-QDVDQREAPLN, from the internal region of the protein sequence according to NP_005154.2; NP_001014431.1; NP_001014432.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in MCF7 cell lysates and approx. 55kDa in preliminary testing of K562 and NIH3T3 cell lysate (calculated MW of 55.7kDa according to NP_005154.2). These molecular weights are observed by other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the microtubules/cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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