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Goat Anti-COL4A3BP (aa662-672) Antibody (EB08147)

Related Products: EBP08147 - Immunizing Peptide, EBP08911 - Immunizing Peptide, Goat Anti-COL4A3BP (aa396-411) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08147 Goat Anti-COL4A3BP (aa662-672) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08147 1
EB08147 (0.5µg/ml) staining of Mouse Heart lysate (A) + peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: COL4A3BP, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein, CERT, CERTL, FLJ20597, GPBP, STARD11, Goodpasture antigen-binding protein, START domain containing 11, alpha 3 type IV collagen binding protein, ceramide transporter
Official Symbol: CERT1
Accession Number(s): NP_005704.1; NP_112729.1
Human GeneID(s): 10087
Non-Human GeneID(s): 68018 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1)

Immunogen

Peptide with sequence DHDSAPLNNRC, from the internal region of the protein sequence according to NP_005704.1; NP_112729.1 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60-65kDa band observed in Mouse Heart lysates (calculated MW of 71.1kDa according to Mouse NP_075909.1). An additional band of unknown identity was also consistently observed at 25kDa. Both bands were successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse

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