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Goat Anti-CRYAB Antibody (EB08149)

Related Products: EBP08149 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08149 Goat Anti-CRYAB Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08149 1
EB08149 (0.05µg/ml) staining of Mouse (A) and Rat (B) Eye lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CRYAB, crystallin, alpha B, CRYA2, CTPP2, HSPB5, heat-shock 20 kD like-protein
Official Symbol: CRYAB
Accession Number(s): NP_001876.1
Human GeneID(s): 1410
Non-Human GeneID(s): 12955 (mouse), 25420 (rat)

Immunogen

Peptide with sequence C-RLEKDRFSVNLD, from the internal region of the protein sequence according to NP_001876.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 22kDa band observed in Mouse and Rat Eye lysates (calculated MW of 20.1kDa according to Mouse NP_034094.1 and Rat NP_037067.1). Recommended concentration: 0.03-0.1-µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

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