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Goat Anti-PRRX1 Antibody (EB08175)

Related Products: EBP08144 - Immunizing Peptide, EBP08175 - Immunizing Peptide, Goat Anti-PRRX1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08175 Goat Anti-PRRX1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08175 1
EB08175 (2µg/ml) staining of LNCaP cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08175 2
EB08175 (1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRRX1, paired related homeobox 1, PHOX1, PMX1, PRX1, homeobox protein PHOX1, paired mesoderm homeo box 1, paired mesoderm homeobox 1
Official Symbol: PRRX1
Accession Number(s): NP_008833.1; NP_073207.1
Human GeneID(s): 5396
Non-Human GeneID(s): 18933 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_008833.1; NP_073207.1).

Immunogen

Peptide with sequence C-GSDTPQQDNDQ, from the internal region of the protein sequence according to NP_008833.1; NP_073207.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 28kDa band observed in Mouse Heart lysates, and approx. 22-23kDa in lysates of cell line LNCaP (calculated MW of 24.4kDa according to Human NP_008833.1) and 27.3kDa according to Mouse NP_035257.1 (.Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Cow

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