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Goat Anti-Proenkephalin Antibody (EB08195)

Related Products: EBP08195 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08195 Goat Anti-Proenkephalin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB08195 1
EB08195 (0.3µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08195 2
EB08195 (0.3µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, Cy3-staining. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
Click here to download PDF datasheet.

Target Protein

Principal Names: proenkephalin, PENK
Official Symbol: PENK
Accession Number(s): NP_006202.1
Human GeneID(s): 5179

Immunogen

Peptide with sequence C-RSHHQDGSDNEE, from the internal region of the protein sequence according to NP_006202.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 37kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrinology. 1998 Apr;139(4):2137-45; PMID: 9529003). Recommended concentration: 1-3µg/ml.
IHC: Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in IHC in Human:
Skrapits K, Borsay BÁ, Herczeg L, Ciofi P, Liposits Z, Hrabovszky E.
Neuropeptide co-expression in hypothalamic kisspeptin neurons of laboratory animals and the human.
Front Neurosci. 2015 Feb 10;9:29.
PMID: 25713511

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