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Code Name Applications Availability Size Tested species Price Grade  
EB08518 Goat Anti-GRIA4 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08518 1
EB08518 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08518 2
EB08518 (2µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRIA4, glutamate receptor, ionotrophic, AMPA 4, GLUR4, GLUR4C, GLURD, AMPA-selective glutamate receptor 4
Official Symbol: GRIA4
Accession Number(s): NP_000820.3; NP_001070711.2; NP_001070712.1
Human GeneID(s): 2893
Non-Human GeneID(s): 14802 (mouse), 29629 (rat)
Important Comments: This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.

Immunogen

Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3; NP_001070711.2; NP_001070712.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx.100kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2kDa according to NP_001070711.2). Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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