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Goat Anti-Dual oxidase 1 Antibody (EB08519)

Related Products: EBP08519 - Immunizing Peptide, EBP11090 - Immunizing Peptide, Goat Anti-DUOX1 (aa972-985) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08519 Goat Anti-Dual oxidase 1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08519 1
EB08519 (4µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DUOX1, dual oxidase 1, LNOX1, MGC138840, MGC138841, NOXEF1, THOX1, NADPH thyroid oxidase 1, flavoprotein NADPH oxidase, nicotinamide adenine dinucleotide phosphate oxidase
Official Symbol: DUOX1
Accession Number(s): NP_059130.2 ; NP_787954.1
Human GeneID(s): 53905
Important Comments: This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical;

Immunogen

Peptide with sequence EKFQRSCLHQTVQ, from the internal region of the protein sequence according to NP_059130.2 ; NP_787954.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in Human Placenta and Thyroid Gland lysates gave no specific signal but low background (at antibody concentration up to 0.5µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Thyroid shows staining of membranes in epithelial cells of the gland. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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