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Goat Anti-Clusterin / ApoJ (mouse) Antibody (EB08920)

Related Products: EBP08920 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08920 Goat Anti-Clusterin / ApoJ (mouse) Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08920 1
EB08920 (0.1µg/ml) staining of Mouse (A) and (0.3µg/ml) Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08920 2
EB08920 Immunofluorescence analysis of paraformaldehyde fixed PC12 suspension cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ApoJ, clusterin, Clu, AI893575, Cli, D14Ucla3, SP-40, Sgp-2, Sgp2, Sugp-2, Apolipoprotein J, complement lysis inhibitor, testosterone repressed prostate message
Official Symbol: Clu
Accession Number(s): NP_038520.2
Non-Human GeneID(s): 12759 (mouse), 24854 (rat)

Immunogen

Peptide with sequence C-EKALQEYRRKSRAE, from the C Terminus of the protein sequence according to NP_038520.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 38kDa band observed in Mouse and Rat Brain lysates.(calculated MW of 51.7kDa according to Mouse NP_038520.2 and 51.4kDa according to Rat XP_006252156.1). This molecular weight is routinely observed by other sources and thought to correspond to the alpha chain of APOJ. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of PC12 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat

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