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Code Name Applications Availability Size Tested species Price Grade  
EB08932 Goat Anti-SEPT6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB08932 1
EB08932 (0.1µg/ml) staining of Daudi (A), Jurkat (B) and MOLT4 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08932 2
EB08932 (1µg/ml) staining of Human Testis (A), (0.1ug/ml) Duodenum (B), (0.5ug/ml) Kidney (C) and (0.3ug/ml) Tonsil (D) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08932 3
EB08932 (3.8µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SEPT6, septin 6, KIAA0128, MGC16619, MGC20339, SEP2, SEPT2, septin 2
Official Symbol: SEPTIN6
Accession Number(s): NP_665798.1; NP_055944.2; NP_665801.1
Human GeneID(s): 23157
Important Comments: This antibody is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.

Immunogen

Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1; NP_055944.2; NP_665801.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow, Dog

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