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Code Name Applications Availability Size Tested species Price Grade  
EB09032 Goat Anti-SODD Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09032 1
EB09032 (1µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09032 2
EB09032 (3.75µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BAG4, BCL2-associated athanogene 4, BAG-4, SODD, BAG-family molecular chaperone regulator-4, silencer of death domains
Official Symbol: BAG4
Accession Number(s): NP_004865.1
Human GeneID(s): 9530

Immunogen

Peptide with sequence C-GNATSDHPNNQDQSS, from the internal region of the protein sequence according to NP_004865.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 60kDa band observed in cell lines HEK293 and HeLa lysates (calculated MW of 49.6kDa according to NP_004865.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Takada et al, Mol Cell Biol. 2003 Jun;23(11):4026-33.; PMID: 12748303). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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