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Goat Anti-GABRB3 Antibody (EB09097)

Related Products: EBP09097 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09097 Goat Anti-GABRB3 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09097 1
EB09097 (2.5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GABRB3, gamma-aminobutyric acid (GABA) A receptor, beta 3, MGC9051, GABA(A) receptor beta-3 subunit, GABA-A receptor beta-3 subunit, GABA-alpha receptor beta-2 subunit, GABAA receptor beta-3 subunit
Official Symbol: GABRB3
Accession Number(s): NP_000805.1; NP_068712.1
Human GeneID(s): 2562
Non-Human GeneID(s): 14402 (mouse), 24922 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1).

Immunogen

Peptide with sequence C-EKTAKAKNDRSK, from the internal region of the protein sequence according to NP_000805.1; NP_068712.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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