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Goat Anti-Desmin Antibody (EB09106)

Related Products: EBP09106 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09106 Goat Anti-Desmin Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09106 1
EB09106 (0.01µg/ml) staining of Human Skeletal Muscle (A) and (0.3ug/ml) Heart (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09106 2
EB09106 (0.1µg/ml) staining of Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09106 3
EB09106 (6µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB09106 4
EB09106 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DES, desmin, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein
Official Symbol: DES
Accession Number(s): NP_001918.3
Human GeneID(s): 1674
Non-Human GeneID(s): 13346 (mouse), 64362 (rat)

Immunogen

Peptide with sequence C-RDGEVVSEATQQQHE, from the C Terminus of the protein sequence according to NP_001918.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 55-60kDa band observed in Human and Mouse Skeletal Muscle lysates and in Human Heart lysates (calculated MW of 53.5kDa according to Human NP_001918.3, and Mouse NP_034173.1). Preliminary testing showed a band at approx 60kDa in Rat and Pig Skeletal muscle lysate. Recommended concentration: 0.01-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Heart. Recommended concentration: 4-6µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog, Cow

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