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Goat Anti-WIZ Antibody (EB09114)

Related Products: EBP09114 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09114 Goat Anti-WIZ Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09114 1
EB09114 (1µg/ml) staining of Human Cerebellum lysate (A) + Blocking peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09114 2
EB09114 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: WIZ, widely interspaced zinc finger motifs, ZNF803, WIZ zinc finger, widely-interspaced zinc finger motifs
Official Symbol: WIZ
Accession Number(s): NP_067064.2; NP_001317324.1
Human GeneID(s): 58525
Non-Human GeneID(s): 22404 (mouse), 314598 (rat)

Immunogen

Peptide with sequence C-RSYIQGGRPFTKKFR, from the internal region of the protein sequence according to NP_067064.2; NP_001317324.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 75kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 85.4kDa according to NP_067064.2). An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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