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Goat Anti-AREB6 / ZEB1 Antibody (EB09166)

Related Products: EBP09166 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09166 Goat Anti-AREB6 / ZEB1 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09166 1
EB09166 (0.05µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09166 2
EB09166 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ZEB1, AREB6, zinc finger E-box binding homeobox 1, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, OTTHUMP00000019405, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding protein
Official Symbol: ZEB1
Accession Number(s): NP_001121600.1; NP_110378.3
Human GeneID(s): 6935
Non-Human GeneID(s): 21417 (mouse), 25705 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3).

Immunogen

Peptide with sequence C-RPQIRQKIENKPLQE, from the internal region of the protein sequence according to NP_001121600.1; NP_110378.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx.100kDa band observed in Mouse Heart lysates (calculated MW of 122kDa according to Human NP_110378.3 and Mouse NP_035676.1). Recommended concentration: 0.05-0.2µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human and Rat Heart for this particular batch.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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