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Goat Anti-DHX9 / RHA Antibody (EB09297)

Related Products: EBP09297 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09297 Goat Anti-DHX9 / RHA Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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www.arp1.com
EB09297 1
EB09297 (1µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DHX9, DEAH (Asp-Glu-Ala-His) box polypeptide 9, DDX9, LKP, NDHII, RHA, ATP-dependent RNA helicase A, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNA helicase II, leukophysin), DEAD/H box-9 (nuclear DNA helicase II, RNA helicase A), OTTHUMP00000033287, leukophysin
Official Symbol: DHX9
Accession Number(s): NP_001348.2
Human GeneID(s): 1660
Non-Human GeneID(s): 13211 (mouse), 304859 (rat)

Immunogen

Peptide with sequence TEGRNALIHKSSVNC , from the internal region of the protein sequence according to NP_001348.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 140kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 141kDa according to NP_001348.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

Specific Reference

This antibody has been successfully used in WB:
Saravana Selvanathan and Jeffrey Toretsky
An EWS::FLI1 monoclonal antibody with multimodal utility and specificity for FLI1.
bioRxiv preprint doi: https://doi.org/10.1101/2023.11.13.566886
PMID: 0

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