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Code Name Applications Availability Size Tested species Price Grade  
EB09512 Goat Anti-Restin / CLIP1 Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09512 1

EB09512 optimised QC. Primary incubation 1 hour at room temperature.

Image A: HEK293 cell lysate at primary Ab concentration 0.1ug/ml, Images B, C: HepG2, A549 cell lysate at primary Ab concentration 0.3ug/ml, and Image D: HeLa cell lysate at primary Ab concentration 1ug/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB09512 2
EB09512 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09512 3
EB09512 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09512 4
EB09512 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB09512 5
EB09512 (2.5µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09512 6
EB09512 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CLIP1, CAP-GLY domain containing linker protein 1, CLIP, CLIP-170, CLIP170, CYLN1, MGC131604, RSN, Reed-Steinberg cell-expressed intermediate filament-associated protein, cytoplasmic linker 1, restin, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
Official Symbol: CLIP1
Accession Number(s): NP_002947.1; NP_937883.1; NP_001234926.1; NP_001376220.1
Human GeneID(s): 6249
Non-Human GeneID(s): 56430 (mouse), 65201 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).

Immunogen

Peptide with sequence C-PHSTHHGSRGEERP, from the C Terminus of the protein sequence according to NP_002947.1; NP_937883.1; NP_001234926.1; NP_001376220.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 150kDa band observed in lysates of cell lines HEK293, A549, HepG2 and HeLa (calculated MW of 161kDa according to NP_002947.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Thyroid and Prostate. Recommended concentration: 2.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in A431 and A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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