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Code Name Applications Availability Size Tested species Price Grade  
EB09512 Goat Anti-Restin / CLIP1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09512 1
EB09512 (1µg/ml) staining of Human Breast Cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09512 2
EB09512 (2.5µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09512 3
EB09512 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CLIP1, CAP-GLY domain containing linker protein 1, CLIP, CLIP-170, CLIP170, CYLN1, MGC131604, RSN, Reed-Steinberg cell-expressed intermediate filament-associated protein, cytoplasmic linker 1, restin, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
Official Symbol: CLIP1
Accession Number(s): NP_002947.1; NP_937883.1
Human GeneID(s): 6249
Non-Human GeneID(s): 56430 (mouse), 65201 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).

Immunogen

Peptide with sequence C-PHSTHHGSRGEERP, from the C Terminus of the protein sequence according to NP_002947.1; NP_937883.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 160kDa band observed in Human Breast Cancer lysates (calculated MW of 161kDa according to NP_002947.1. Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Thyroid and Prostate. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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