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Goat Anti-DTX3 Antibody (EB09706)

Related Products: EBP09706 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09706 Goat Anti-DTX3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Mouse, Human $411.00 Elite

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09706 1
EB09706 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09706 2
EB09706 (10µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DTX3, deltex 3 homolog (Drosophila), FLJ34766, MGC138863, MGC138864, RNF154, deltex 3 homolog
Official Symbol: DTX3
Accession Number(s): NP_848597.1
Human GeneID(s): 196403
Non-Human GeneID(s): 80904 (mouse), 500847 (rat)

Immunogen

Peptide with sequence C-KNKVTVSKPVWD, from the internal region (near N Terminus) of the protein sequence according to NP_848597.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13;278(24):21930-7.; PMID: 12670957). Recommended concentration: 0.2-0.5µg/ml.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Mouse, Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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