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Goat Anti-SNX1 Antibody (EB09792)

Related Products: EBP09792 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09792 Goat Anti-SNX1 Antibody Pep-ELISA, WB, IHC, ICC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Email: [email protected]
www.arp1.com
EB09792 1
EB09792 (1µg/ml) staining of HeLa (A) and (0.1ug/ml) U2OS (B) cell lysate (35µg protein in RIPA buffer).. Detected by chemiluminescence.
EB09792 2
EB09792 (3.8µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09792 3
EB09792 (5ug/ml) staining of formaldehyde-fixed SH5Y5Y and detected with Alexa 488 in confocal microscopy. Data obtained from Dr. Schallburg Nielsen, Aarhus University Denmark.
Click here to download PDF datasheet.

Target Protein

Principal Names: HsT17379, MGC8664, SNX1A, sorting nexin 1, sorting nexin 1A, Vps5, SNX1
Official Symbol: SNX1
Accession Number(s): NP_003090.2; NP_690039.1
Human GeneID(s): 6642
Non-Human GeneID(s): 56440 (mouse), 84471 (rat)
Important Comments: This antibody is expected to recognize isoform a and c (NP_003090.2; NP_690039.1.

Immunogen

Peptide with sequence C-TNSSKPQPTYEELE, from the internal region of the protein sequence according to NP_003090.2; NP_690039.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 75kDa band observed in lysates of cell lines HeLa and U2OS (calculated MW of 59.1kDa according to NP_003090.2). This molecular weight is routinely observed by other sources . Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Uterus shows vesicular staining in the cytoplasm of endometrial epithelium. Recommended concentration, 3-5µg/ml.

Immunocytochemistry: Vesicular staining of SNX1 (green) in formaldehyde-fixed SH5Y5Y. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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