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Code Name Applications Availability Size Tested species Price Grade  
EB09839 Goat Anti-CHCHD3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09839 1
EB09839 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09839 2
EB09839 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB09839 3
EB09839 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: coiled-coil-helix-coiled-coil-helix domain containing 3, FLJ20420, CHCHD3
Official Symbol: CHCHD3
Accession Number(s): NP_060282.1
Human GeneID(s): 54927
Non-Human GeneID(s): 296966 (rat)

Immunogen

Peptide with sequence KFKRYESHPVCAD, from the internal region of the protein sequence according to NP_060282.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum) and Heart lysates (calculated MW of 26.2kDa according to NP_060282.1). Recommended concentration: 1-3µg/ml. A customer found ~30kDa in THP1 and in Human primary monocyte derived dendritic cells.
IHC: Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep

Specific Reference

This antibody has been successfully used in IF on Human:
Steffen J, Vashisht AA, Wan J, Jen JC, Claypool SM, Wohlschlegel J, Koehler CM.
Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2 mediated hyperfusion of mitochondria.
Mol Biol Cell. 2017 Jan 5.
PMID: 28057766

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