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Goat Anti-TRIM71 (aa390-402) Antibody (EB09901)

Related Products: EBP09901 - Immunizing Peptide, EBP09902 - Immunizing Peptide, Goat Anti-TRIM71 (aa409-419) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09901 Goat Anti-TRIM71 (aa390-402) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09901 1
EB09901 (0.3µg/ml) staining of HEK293 (A) and NIH3T3 (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09901 2
EB09901 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: abnormal cell LINeage LIN-41, homolog of C. elegans Lin-41, LIN41, LIN-41, tripartite motif-containing 71, TRIM71
Official Symbol: TRIM71
Accession Number(s): NP_001034200.1
Human GeneID(s): 131405
Non-Human GeneID(s): 636331 (mouse), 301042 (rat)

Immunogen

Peptide with sequence C-RQVKAKSLYLQVE, from the internal region of the protein sequence according to NP_001034200.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and 92.1kDa according to Mouse NP_001035968.1). The observed molecular weight corresponds to findings with EB09902, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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