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Goat Anti-Myogenin / MYF4 Antibody (EB09955)

Code Name Applications Availability Size Tested species Price Grade  
EB09955 Goat Anti-Myogenin / MYF4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $423.00 Elite

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EB09955 1
EB09955 (1µg/ml) staining of Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: bHLHc3, MYF4, myogenic factor 4, Myogenic factor-4, MYOGENIN, myogenin, myogenin, myogenin (myogenic factor 4), MYOG
Official Symbol: MYOG
Accession Number(s): NP_002470.2
Human GeneID(s): 4656

Immunogen

Peptide with sequence C-TDAHNLHSLTSIVD, from the C Terminus (near) of the protein sequence according to NP_002470.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 25.2kDa according to Mouse NP_112466.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig