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Goat Anti-SLAMF8 Antibody (EB10495)

Related Products: EBP10495 - Immunizing Peptide, EBP10496 - Immunizing Peptide, Goat Anti-SLAMF8 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10495 Goat Anti-SLAMF8 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10495 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human SLAMF8 with C-terminal MYC tag probed with EB10495 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10495 (1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
EB10495 2
EB10495 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: B lymphocyte activator macrophage expressed, BCM-like membrane protein, BLAME, FLJ20442, MGC129578, SBBI42, SLAM family member 8, SLAMF8
Official Symbol: SLAMF8
Accession Number(s): NP_064510.1
Human GeneID(s): 56833
Non-Human GeneID(s): 74748 (mouse), 289237 (rat)

Immunogen

Peptide with sequence C-TLYHSRFLGRAQ, from the internal region of the protein sequence according to NP_064510.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: In transfected HEK293 transiently expressing Human SLAMF8 (myc and DYKDDDDK tagged), a band of approx. 45kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration: 0.5-1µg/ml.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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