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Goat Anti-COPA Antibody (EB10513)

Related Products: EBP10513 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10513 Goat Anti-COPA Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10513 1
EB10513 (0.5µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: alpha coat protein, coatomer protein complex, subunit alpha, FLJ26320, HEP-COP, xenin, COPA
Official Symbol: COPA
Accession Number(s): NP_001091868.1; NP_004362.2
Human GeneID(s): 1314
Non-Human GeneID(s): 12847 (mouse), 304978 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001091868.1; NP_004362.2).

Immunogen

Peptide with sequence CTRASNLENST, from the internal region of the protein sequence according to NP_001091868.1; NP_004362.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 140kDa band observed in Human Prostate lysates (calculated MW of 138kDa according to NP_001091868.1and NP_004362.2). Recommended concentration: 0.5-2µg/ml. An additional band of 52kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

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