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Code Name Applications Availability Size Tested species Price Grade  
EB10644 Goat Anti-RAN (aa 197-210) Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10644 1
EB10644 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10644 2
EB10644 (0.1µg/ml) staining of Mouse (A) and Rat (B) Testis lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10644 3
EB10644 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: RAN, RAN, member RAS oncogene family, ARA24, Gsp1, TC4, GTP-binding nuclear protein Ran, GTPase Ran, OK/SW-cl.81, OTTHUMP00000166078, RanGTPase, androgen receptor-associated protein 24, guanosine triphosphatase Ran, member RAS oncogene
Official Symbol: RAN
Accession Number(s): NP_006316.1
Human GeneID(s): 5901
Non-Human GeneID(s): 19384 (mouse), 84509 (rat)

Immunogen

Peptide with sequence C-YEHDLEVAQTTALP, from the C Terminus of the protein sequence according to NP_006316.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 25kDa band observed in Human, Mouse and Rat Testis lysates (calculated MW of 24.4kDa according to NP_006316.1). Recommended concentration: 0.1-1µg/ml. Primary incubation was 1 hour.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of A431 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Cow

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