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Goat Anti-Gsta3/Gsta5/Gst Yc2 (rat) Antibody (EB10727)

Code Name Applications Availability Size Tested species Price Grade  
EB10727 Goat Anti-Gsta3/Gsta5/Gst Yc2 (rat) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $423.00 Elite

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EB10727 1
EB10727 (0.3µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Gsta5, glutathione S-transferase Yc2 subunit, MGC112704, Yc2, GST A5-5, GST Yc2, glutathione S-transferase Yc-2, glutathione S-transferase alpha-5, glutathione S-transferase A3, Gsta3, glutathione S-transferase alpha 3, glutathione S-transferase alpha 3
Official Symbol: Gsta3
Accession Number(s): NP_001009920.1
Non-Human GeneID(s): 14859 (mouse), 494500 (rat)
Important Comments: Reported variants represent identical protein: NP_001009920.1, NP_001153211.1. Please note that this antibody is expected to cross-react to the homologous Mouse protein Gsta3

Immunogen

Peptide with sequence C-EMDPGIVDNFPLLK, from the internal region of the protein sequence according to NP_001009920.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 25kDa band observed in Mouse and Rat lysates (calculated MW of 25.3kDa according to NP_001009920.1. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat