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Code Name Applications Availability Size Tested species Price Grade  
EB10960 Goat Anti-CB1 (aa44-58) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10960 1
EB10960 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: CNR1, cannabinoid receptor 1 (brain), CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR, cannabinoid receptor 1, central cannabinoid receptor
Official Symbol: CNR1
Accession Number(s): NP_057167.2; NP_149421.2
Human GeneID(s): 1268
Non-Human GeneID(s): 12801 (mouse), 25248 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_057167.2; NP_149421.2). Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1

Immunogen

Peptide with sequence C-NITEFYNKSLSSFKE, from the internal region (near N Terminus) of the protein sequence according to NP_057167.2; NP_149421.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70+55kDa bands observed in Rat Brain lysates (calculated MW of 52.9kDa according to Human NP_057167.2 and 52.8kDa according to Rat NP_036916.1).The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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