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Goat Anti-Tgm6 (mouse) Antibody (EB11119)

Code Name Applications Availability Size Tested species Price Grade  
EB11119 Goat Anti-Tgm6 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $423.00 Elite

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EB11119 1
EB11119 (2µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: A030011P16, OTTMUSP00000016517, protein-glutamine gamma-glutamyltransferase 6, RP23-20A6.2, TGM3L, TGY, transglutaminase 6, Tgm6
Official Symbol: Tgm6
Accession Number(s): NP_808394.1; NP_001276676.1
Human GeneID(s): 343641
Non-Human GeneID(s): 241636 (mouse), 296152 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_808394.1; NP_001276676.1). Variants NP_001276676.1, NP_001276677.1 and NP_001276678.1 represent identical protein.

Immunogen

Peptide with sequence C-DKYVDSYGRTLED, from the C Terminus of the protein sequence according to NP_808394.1; NP_001276676.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in Rat Lung lysates (calculated MW of 79.3kDa according to Mouse NP_945345.2 and 80.4kDa according to Rat XP_230601.5). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow