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Code Name Applications Availability Size Tested species Price Grade  
EB11231 Goat Anti-SOX11 (aa309-323) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11231 1
EB11231 (0.5µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11, SOX11
Official Symbol: SOX11
Accession Number(s): NP_003099.1
Human GeneID(s): 6664
Non-Human GeneID(s): 20666 (mouse), 84046 (rat)

Immunogen

Peptide with sequence C-SRLYYSFKNITKQHP, from the internal region of the protein sequence according to NP_003099.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 65kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 46.7kDa according to NP_003099.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Dictor et al, Haematologica. 2009 Nov;94(11):1563-8. PMID: 19880779). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3, Mouse and Rat Brain for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

Specific Reference

This antibody has been successfully used in IHC on Mouse:
Chen C, Jin J, Lee GA, Silva E, Donoghue M.
Cross-species functional analyses reveal shared and separate roles for Sox11 in frog primary neurogenesis and mouse cortical neuronal differentiation.
Biol Open. 2016 Apr 15;5(4):409-17
PMID: 26962049

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